Human coronavirus HKU1 (isolate N2) Hemagglutinin-esterase (His)

Human coronavirus HKU1 (isolate N2) Hemagglutinin-esterase (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 45.9 kDa and the accession number is Q14EB1.

规格	价格/CNY		货期	数量
20 μg	￥ 12,800	20日内发货
100 μg	￥ 19,800	20日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 12,800

20日内发货

100 μg

￥ 19,800

20日内发货

产品描述	Human coronavirus HKU1 (isolate N2) Hemagglutinin-esterase (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 45.9 kDa and the accession number is Q14EB1.

种属	HCoV-HKU1
表达系统	E. coli
标签	N-10xHis
蛋白编号	Q14EB1
别名	HE, E3 glycoprotein, Hemagglutinin-esterase
氨基酸序列	YGFNEPLNVVSHLNHDWFLFGDSRSDCNHINNLKIKNYGYLDIHPSLCNNGKISSSAGDSIFKSYHFTRFYNYTGEGDQIIFYEGVNFNPHHRFKCFFNGSNDVWIFNKVRFYRALYSNMALFRYLTFVDILYNFSFSIKANICNSNILSLNNPIFISTNYSKDVYFTLSGCSLYLVPLCLFKSNFSQYYYNMDTGFAYGYSNFVSSDLDCTYISLKPGSYKIFSTGFVLSIPTKALCFNKSKQFVPVQVVDSRWNNLRASDTSLSDACQLPYCYFRNSSGNYVGKYDINHGDNGFTSILSGLLYNVSCISYYGSFLYDNFTSIWPRFSFGNCPTSAYIKLNCFYDPLPIILQGILLFLALLFIVFLLFLVYHG
蛋白构建	12-385 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	45.9 kDa (predicted)

缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.

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Human coronavirus HKU1 (isolate N2) Hemagglutinin-esterase (His)

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