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Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 1,920 | 5日内发货 | |
500 μg | ¥ 7,710 | 5日内发货 | |
1 mg | ¥ 12,800 | 5日内发货 |
生物活性 | Immobilized Human EpCAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. |
产品描述 | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P16422 |
别名 | TROP1,TACSTD1,TACST-1,MOC31,MK1,MIC18,KSA,HNPCC8,gp40,ESA,EpCAM,EGP40,EGP314,EGP-2,EGP,DIAR5,CD326,ACSTD1 |
蛋白构建 | Gln24-Lys265 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 28.2 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
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