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Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | |
500 μg | ¥ 12,400 | 5日内发货 | |
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Immobilized Human EFEMP1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA. |
产品描述 | Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-His |
蛋白编号 | Q12805-1 |
别名 | S1-5,MTLV,MLVT,MGC111353,Fibulin 3,fibrillin-like,FIBL-3,FBNLFLJ35535,FBNL,FBLN3,EFEMP1,DHRD |
蛋白构建 | Gln18-Phe493 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 53.9 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. |
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