DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 42.1 kDa and the accession number is Q47YI0.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 1,510 | 20日内发货 | ||
100 μg | ¥ 2,840 | 20日内发货 | ||
1 mg | ¥ 12,200 | 20日内发货 |
产品描述 | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 42.1 kDa and the accession number is Q47YI0. |
种属 | Colwellia psychrerythraea |
表达系统 | E. coli |
标签 | N-10xHis |
蛋白编号 | Q47YI0 |
别名 | ligA, DNA ligase, Polydeoxyribonucleotide synthase [NAD(+)] |
氨基酸序列 | KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI |
蛋白构建 | 330-689 aa |
蛋白纯度 | > 85% as determined by SDS-PAGE. |
分子量 | 42.1 kDa (predicted) |
缓冲液 | Tris-based buffer, 50% glycerol |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) ligA DNA ligase Polydeoxyribonucleotide synthase [NAD(+)] recombinant recombinant-proteins proteins protein