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CLPS Protein, Human, Recombinant (His)

产品编号 TMPY-02846

别名: colipase, pancreatic

CLPS Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 11.5 kDa and the accession number is P04118.

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CLPS Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		现货
200 μg	￥ 6,530		5日内发货
500 μg	￥ 13,220		5日内发货

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生物活性	Testing in progress
产品描述	CLPS Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 11.5 kDa and the accession number is P04118.

种属	Human
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	P04118
别名	colipase, pancreatic
蛋白构建	The Human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	11.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 500 mM NaCl, pH 7.0, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

参考文献

1. Davis RC, et al. (1991) Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter. Genomics. 10(1):262-5. 2. Lowe ME. (1997) Structure and function of pancreatic lipase and colipase. Annu Rev Nutr. 17: 141-58. 3. Verger R, et al. (1999) Colipase: structure and interaction with pancreatic lipase. Biochim Biophys Acta. 1441(2-3):173-84.

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Keywords

CLPS Protein, Human, Recombinant (His) colipase, pancreatic recombinant recombinant-proteins proteins protein

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