Green DND-26 is a lysosomal probe tagged with green fluorescence, displaying peak excitation and emission wavelengths at 504/511 nm. It consists of a fluorescein group and attached weak bases that can easily permeate the cell membrane and predominantly accumulate in spherical organelles. This compound is effective for examining lysosomal biosynthesis and associated pathogenesis [1].

Green DND-26工作液配置：将溶液恢复至室温，瞬时离心至管底。使用培养基或合适的缓冲液（如PBS）将1 mM储存液稀释至工作浓度，推荐浓度为50-100 nM。请根据实际情况调整Green DND-26工作液浓度，需现用现配。细胞染色：悬浮细胞以1000 g, 4°C离心收集，加PBS洗涤两次，每次5分钟；贴壁细胞弃去培养基，加入胰蛋白酶消化，离心弃去上清，加PBS洗涤两次，每次5分钟。加入1 mL Green DND-26工作液，在室温孵育30分钟。以400 g，4°C离心。