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CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant

产品编号 TMPY-03328
CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.7 kDa and the accession number is G7NCB9.
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
CD3D & CD3E Heterodimer Protein, Rhesus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 76.7 kDa and the accession number is G7NCB9.
种属
Rhesus
表达系统
HEK293 Cells
标签Tag Free
蛋白编号G7NCB9
别名
CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex)
蛋白构建
A DNA sequence encoding the rhesus CD3D extracellular domain (F6WI60)(Met1-Ala105) with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rhesus CD3E extracellular domain(XP_014971302.1) (Met1-Asp117) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified. Predicted N terminal: Gln 22 & Phe 22
蛋白纯度
> 95 % as determined by SDS-PAGE
分子量76.7 kDa (predicted); 52 & 43 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.

计算器

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  • 重组蛋白稀释 计算器
  • 比活力 计算器

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请阅读 重组蛋白用户指南 了解更多具体信息.

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