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Anti-PSAP Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF.
Anti-PSAP Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μg | ¥ 2,087.42¥ 3,599 | 7日内发货 | |
50 μg | ¥ 3,478.84¥ 5,998 | 7日内发货 | |
100 μg | ¥ 5,218.26¥ 8,997 | 7日内发货 |
产品描述 | Anti-PSAP Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting PSAP. Anti-PSAP Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF. |
别名 | Prosaposin, Proactivator polypeptide |
Ig Type | Rabbit monoclonal IgG |
交叉反应 | Human |
验证活性 | Flow cytometry analysis of PSAP overexpressed 335F cells with TMAZ-0240A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
应用 | ELISA, FACS, IF |
推荐剂量 | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted |
构建方式 | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of PSAP protein. |
纯化方式 | Protein A Affinity Purified |
性状 | Liquid |
缓冲液 | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
纯度 | > 95% as determined by SDS-PAGE. |
研究背景 | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate|Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases|Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12)|Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling|Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases |
偶联 | AF647 |
其他可选修饰符 | Biotin/APC/PE/AF488/AF555 |
可提供抗体种类 | 3 |
免疫原 | PSAP |
抗原种属 | Human |
基因ID | 5660 |
Uniprot ID | |
研究领域 | Other Functions |
分子量 | 150 kDa |
储存方式 | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
运输方式 | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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