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Anti-LCAT Monoclonal Antibody-AF555

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产品编号 TMAZ-0161A5
别名 Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase

Anti-LCAT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-AF555 can be used in ELISA, FACS.

Anti-LCAT Monoclonal Antibody-AF555

Anti-LCAT Monoclonal Antibody-AF555

Rating icon 还可以
产品编号 TMAZ-0161A5 别名 Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase

Anti-LCAT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-AF555 can be used in ELISA, FACS.

规格价格库存数量
25 μg¥ 3,5997日内发货
50 μg¥ 5,9987日内发货
100 μg¥ 8,9977日内发货
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产品介绍

生物活性
产品描述
Anti-LCAT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-AF555 can be used in ELISA, FACS.
别名Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase
Ig Type
Rabbit monoclonal IgG
交叉反应
Human
验证活性
Flow cytometry analysis of LCAT overexpressed 431F cells with TMAZ-0161A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line).
应用
ELISA, FACS
推荐剂量
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Single-pass type I membrane
构建方式This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of LCAT protein.
纯化方式Protein A Affinity Purified
性状Liquid
缓冲液0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
纯度> 95% as determined by SDS-PAGE.
研究背景Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed: 10329423, PubMed: 19065001, PubMed: 26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16: 0-18: 2 or 18: O-18: 2 phosphatidylcholines (PubMed: 8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed: 10722751). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed: 8016111). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (PubMed: 8016111). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (PubMed: 24620755)
偶联与修饰
偶联
AF555
其他可选修饰符
Biotin/APC/PE/AF488/AF647
可提供抗体种类
1
抗原信息
免疫原
LCAT
抗原种属
Human
基因ID
3931
Uniprot ID
研究领域
Drug Target
化学信息
分子量150 kDa
存储&运输
储存方式For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
运输方式For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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