Vaccinia Virus B18R/B19R Protein (His)

B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus. It is also known as B19R in the Copenhagen strain of Vaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.

规格	价格/CNY		数量
100 μg	￥ 4,470	现货
200 μg	￥ 7,630	5日内发货
500 μg	￥ 15,450	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 4,470

现货

200 μg

￥ 7,630

5日内发货

500 μg

￥ 15,450

5日内发货

生物活性

Measured by its ability to neutralize B18R-mediated inhibition of WISH cell pathology, IFNA2 can bind to B18R and counteract its inhibitory effect. The neutralization rate, reflecting the activity of B18R, is calculated by measuring cell viability using the MTT assay. The ED50 for this effect is 0.16-1.2 ng/mL.

产品描述

种属	VACV
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	P25213
蛋白构建	B18R (P25213) (Met1-Glu351)
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	39.86 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	B18R is a type I interferon (IFN)-binding protein, which is encoded by the B18R open reading frame in the WR (Western Reserve) strain of vaccinia virus. It is also known as B19R in the Copenhagen strain of Vaccinia. B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. It has been shown that secreted B18R binds to uninfected and infected cells. It presents at the cell surface and protects cells from the antiviral state induced by IFN-alpha and IFN-beta. Binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.