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Thy1/CD90 Protein, Human, Recombinant (His)

产品编号 TMPY-05087

别名: Thy-1 cell surface antigen, CD90

Thy1/CD90 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is B0YJA4.

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Thy1/CD90 Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 2,270		5日内发货
500 μg	￥ 14,800		5日内发货

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参考文献

生物活性	Loaded Recombinant Human Thy1/CD90 Protein, His Tag on NTA Biosensor, can bind Recombinant Human Galectin-1 Protein with an affinity constant of 46.9 nM as determined in BLI assay (Routinely tested).
产品描述	Thy1/CD90 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14 kDa and the accession number is B0YJA4.

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	B0YJA4
别名	Thy-1 cell surface antigen, CD90
蛋白构建	The Human THY1 (NP_006279.2) (Met1-Cys130) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
分子量	14 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.

参考文献

1. Rege TA,et al.(2006) Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 20(8): 1045-54. 2. Fiegel HC,et al.(2008) Lack of Thy1 (CD90) expression in neuroblastomas is correlated with impaired survival. Pediatr Surg Int. 24(1): 101-5. 3. Bradley JE,et al.(2009) Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 35(3): 258-65. 4. Kisselbach L,et al.(2009) CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures. Cytotechnology. 59(1): 31-44.

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Keywords

Thy1/CD90 Protein, Human, Recombinant (His) Thy-1 cell surface antigen CD 90 CD-90 CD90 recombinant recombinant-proteins proteins protein

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