PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947.
生物活性 | Testing in progress |
产品描述 | PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947. |
种属 | Human |
表达系统 | E. coli |
标签 | N-His |
蛋白编号 | P17947 |
别名 | SFPI1, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene, PU.1, SPI-1, OF |
蛋白构建 | The Human SPI1 (P17947) (Met1-His270) was expressed, with a polyhistidine tag at the N-terminus. |
蛋白纯度 | ≥ 90 % as determined by SDS-PAGE. |
分子量 | 32.04 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 0.05% SKL. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | SPI1 (also known as PU.1) is a dominant but transient regulator in early T-cell precursors and a potent transcriptional controller of developmentally important pro-T-cell genes. SPI1 is an essential transcription factor (TF) for the hematopoietic lineage, in which its expression is tightly controlled through a -17-kb upstream regulatory region and a promoter region. The E26 transformation-specific (ETS) family transcription factor PU.1/Spi1 acts as a master regulator of myeloid and lymphoid development. PU.1-deficient mice show a complete loss of microglia, indicating that PU.1 plays a pivotal role in micro-gliogenesis. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor. |
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PU.1/SPI1 Protein, Human, Recombinant (His) SFPI1 SPI-A spleen focus forming virus (SFFV) proviral integration oncogene SFPI 1 SFPI-1 SPI1 PU.1 SPI-1 SPI 1 OF recombinant recombinant-proteins proteins protein