MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated

MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 44.1 kDa and the accession number is Q08431.2.

规格	价格/CNY		货期	数量
20 μg	￥ 2,940	5日内发货
100 μg	￥ 6,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,940

5日内发货

100 μg

￥ 6,800

5日内发货

生物活性	Testing in progress
产品描述	MFGE8 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus insect cells with His and Avi tag. The predicted molecular weight is 44.1 kDa and the accession number is Q08431.2.

种属	Human
表达系统	Baculovirus Insect Cells
标签	His, Avi
蛋白编号	Q08431.2
别名	HsT19888, SPAG10, MFGM, MFG-E8, OAcGD3S, milk fat globule-EGF factor 8 protein, hP47, SED1, EDIL1, BA46, HMFG
蛋白构建	The Human MFGE8 (Q08431.2) (Met1-Cys387) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	44.1 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 150 mM NaCl, pH 8.5, 10% glycerol, 0.2 M Arginine, 0.01% Tween-20.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.