ITK Protein, Mouse, Recombinant (aa 351-619, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 58.4 kDa and the accession number is Q03526.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 2,530 | 5日内发货 |
生物活性 | No Kinase Activity |
产品描述 | ITK Protein, Mouse, Recombinant (aa 351-619, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 58.4 kDa and the accession number is Q03526. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | Q03526 |
别名 | Tcsk, IL2-inducible T-cell kinase, Emt, Tsk |
蛋白构建 | The Mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 80 % as determined by SDS-PAGE |
分子量 | 58.4 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 7.5, 10% gly, 0.5 mM GSH. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | IL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family, and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain, and 1 SH3 domain. As an intracellular kinase expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. It regulates the development, function, and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in the regulation of the adaptive immune response. Effects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk of lymphoma. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
ITK Protein, Mouse, Recombinant (aa 351-619, His & GST) Tcsk IL2-inducible T-cell kinase Emt Tsk recombinant recombinant-proteins proteins protein