IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 35.6 kDa and the accession number is A0A024RDA5.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,430 | 5日内发货 | ||
1 mg | ¥ 19,100 | 5日内发货 |
生物活性 | Immobilized Human IL-8/CXCL8 (His Tag) at 2 μg/mL (100 μL/well) can bind Anti-IL-8/CXCL8 Antibody, Mouse Monoclonal, the EC50 is 16-90 ng/mL. |
产品描述 | IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99, hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 35.6 kDa and the accession number is A0A024RDA5. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-hFc |
蛋白编号 | A0A024RDA5 |
别名 | LECT, MONAP, IL-8, LYNAP, 白细胞介素, chemokine (C-X-C motif) ligand 8, GCP-1, Interleukin-8, NAP-1, 白介素, NAF, GCP1, NAP1, IL8, LUCT, MDNCF |
蛋白构建 | The 77 amino acid endothelial-cell derived form ofthe mature Human IL8 (NP_000575.1) (Ala 23-Ser 99) was fused with the Fc region of Human IgG1 at the N-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 35.6 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. |
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IL-8/CXCL8 Protein, Human, Recombinant (aa 23-99, hFc) LECT MONAP IL-8 LYNAP 白细胞介素 chemokine (C-X-C motif) ligand 8 NAP 1 GCP-1 Interleukin-8 NAP-1 白介素 NAF GCP1 GCP 1 NAP1 IL 8 IL8 LUCT MDNCF recombinant recombinant-proteins proteins protein