ICAM-2/CD102 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 50 kDa and the accession number is P35330.
生物活性 | Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically. |
产品描述 | ICAM-2/CD102 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 50 kDa and the accession number is P35330. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | P35330 |
别名 | intercellular adhesion molecule 2, Ly-60, CD102, Icam-2 |
蛋白构建 | The Mouse ICAM2 (NP_034624.1) extracellular domain (Met 1-Gln 222) was fused with the Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 93 % as determined by SDS-PAGE |
分子量 | 50 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris-Citric acid, 150 mM NaCl, pH 5. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As the second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity, and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relationship with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in the diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment. |
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ICAM-2/CD102 Protein, Mouse, Recombinant (hFc) intercellular adhesion molecule 2 Ly-60 CD 102 CD102 Icam-2 CD-102 recombinant recombinant-proteins proteins protein