gp130/IL6ST Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 68 kDa and the accession number is Q00560.
规格 | 价格/CNY | 货期 | 数量 | |
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20 μg | ¥ 690 | 现货 | ||
100 μg | ¥ 1,380 | 5日内发货 |
生物活性 |
1. Measured by its ability to bind mouse IL11Ra in a functional ELISA.
2. Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
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产品描述 | gp130/IL6ST Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 68 kDa and the accession number is Q00560. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q00560 |
别名 | CD130, D13Ertd699e, interleukin 6 signal transducer, gp130, 5133400A03Rik, BB405851, AA389424 |
蛋白构建 | The Mouse gp130 (NP_034690.3) extracellular domain (Met 1-Glu 617) was fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 |
≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
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分子量 | 68 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta, or CD130) is a transmembrane protein that is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively, spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1, and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events. |
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gp130/IL6ST Protein, Mouse, Recombinant (His) CD130 BB-405851 D13Ertd699e interleukin 6 signal transducer AA 389424 gp130 AA-389424 5133400A03Rik BB 405851 CD-130 CD 130 BB405851 AA389424 recombinant recombinant-proteins proteins protein