E-Cadherin/Cadherin-1 Protein, Human, Recombinant (hFc), Biotinylated

E-Cadherin/Cadherin-1 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 87.1 kDa and the accession number is P12830-1.

规格	价格/CNY		货期	数量
20 μg	￥ 1,950	5日内发货
100 μg	￥ 4,620	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 1,950

5日内发货

100 μg

￥ 4,620

5日内发货

生物活性	Testing in progress
产品描述	E-Cadherin/Cadherin-1 Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 87.1 kDa and the accession number is P12830-1.

种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	P12830-1
别名	E-Cadherin, CD324, Arc-1, UVO, E-cad, cadherin 1, type 1, E-cadherin (epithelial), LCAM, CDHE, CDH1, ECAD
蛋白构建	The Human E-Cad (P12830-1)(Met1-Ile707) was expressed with the Fc region of Human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	87.1 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.