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Cyclin E Protein, Mouse, Recombinant (His & GST)

产品编号 TMPY-03535

别名: cyclin E1, AW538188, CycE1

Cyclin E Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 74.8 kDa and the accession number is AAH62152.1.

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Cyclin E Protein, Mouse, Recombinant (His & GST)

规格	价格/CNY		货期	数量
100 μg	￥ 4,460		5日内发货

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参考文献

生物活性	Testing in progress
产品描述	Cyclin E Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 74.8 kDa and the accession number is AAH62152.1.

种属	Mouse
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	AAH62152.1
别名	cyclin E1, AW538188, CycE1
蛋白构建	The Mouse CCNE1 (AAH62152.1) (Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	74.8 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM Nacl, 3 mM DTT, 0.5 mM GSH, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1 / S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

参考文献

1. Honda R, et al. (2005) The structure of cyclin E1 / CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63. 2. Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.

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Keywords

Cyclin E Protein, Mouse, Recombinant (His & GST) cyclin E1 AW-538188 AW 538188 AW538188 CycE1 recombinant recombinant-proteins proteins protein

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