CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
200 μg | ¥ 3,820 | 现货 |
生物活性 | 1 μg = 5 U, 1 U is defined as cutting 1 μg 1 KB dsDNA in 30 min at 37℃ |
产品描述 | CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 163 kDa and the accession number is Q99ZW2. |
种属 | Streptococcus pyogenes |
表达系统 | Baculovirus Insect Cells |
标签 | C-His |
蛋白编号 | Q99ZW2 |
别名 | SpyCas9 |
蛋白构建 | The s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus. |
蛋白纯度 |
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
分子量 | 163 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied from sterile 20 mM Tris, 300 mM NaCl, 50% glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
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CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His) SpyCas9 recombinant recombinant-proteins proteins protein