CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 72.9 kDa and the accession number is Q60737.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 3,170 | 5日内发货 |
生物活性 | The specific activity was determined to be 34 nmol/min/mg using casein as substrate. |
产品描述 | CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 72.9 kDa and the accession number is Q60737. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | Q60737 |
别名 | casein kinase 2, alpha 1 polypeptide, casein kinase 2, α1 polypeptide, CK2 α/CSNK2A1, Csnk2a1-rs4 |
蛋白构建 | The Mouse CSNK2A1 (Q60737) (Met1-Gln391) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 85 % as determined by SDS-PAGE |
分子量 | 72.9 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
研究背景 | Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser / Thr protein kinase family and CK2 subfamily. Casein kinase II (CSNK2A1) is a serine / threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Casein kinase II (CSNK2A1) is a constitutively active, ubiquitously expressed serine / threonine protein kinase that is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CSNK2A1 functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alpha and alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits are catalytically identical, proteins that regulate CSNK2A1, such as cdc2 and Hsp90, preferentially bind to the alpha and not the alpha'-subunit. CSNK2A1 can phosphorylate a number of key intracellular signaling proteins implicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CSNK2A1 is also thought to influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via the phosphorylation of Akt. |
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CK2 alpha/CSNK2A1 Protein, Mouse, Recombinant (His & GST) casein kinase 2, alpha 1 polypeptide casein kinase 2, α1 polypeptide CK2 α/CSNK2A1 casein kinase 2, a 1 polypeptide casein kinase 2, α 1 polypeptide Csnk2a1-rs4 recombinant recombinant-proteins proteins protein