首页工具

登录

Carboxypeptidase B1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01422

别名: carboxypeptidase B1 (tissue), 1810063F02Rik, AI504870, 0910001A18Rik, 2210008M23Rik

Carboxypeptidase B1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is B2RS76.

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

TargetMol

Carboxypeptidase B1 Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 3,820		5日内发货

大包装超大折扣，点击咨询

更多批次查询请联系客服

生物活性

技术参数

产品性质

参考文献

生物活性	Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg. The specific activity is >5,000 pmoles/min/μg.
产品描述	Carboxypeptidase B1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is B2RS76.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	B2RS76
别名	carboxypeptidase B1 (tissue), 1810063F02Rik, AI504870, 0910001A18Rik, 2210008M23Rik
蛋白构建	The Mouse CPB1 (NP_083982.1) precursor (Met 1-Tyr 415) was expressed, with a C-terminal polyhistidine tag.
蛋白纯度	> 94 % as determined by SDS-PAGE
分子量	47.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM MES, 150 mM NaCl, pH 6. 5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

参考文献

1. Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. 2. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. 3. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

=

Mass (in vial)

÷

Desired Reconstitution Concentration

bottom

技术支持

您可能有的问题的答案可以在重组蛋白操作手册中找到

Keywords

Carboxypeptidase B1 Protein, Mouse, Recombinant (His) carboxypeptidase B1 (tissue) AI 504870 1810063F02Rik AI504870 tissue 0910001A18Rik carboxypeptidase B1 AI-504870 2210008M23Rik recombinant recombinant-proteins proteins protein

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Copyright © 2015-2024 TargetMol Chemicals Inc. All rights reserved. 沪ICP备20019793号-4

沪公网安备 31010602006700号

沪公网安备 31010602006700号

TargetMol Loading

陶术

生物

TargetMol^®中国区唯一合作伙伴

点击进入陶术生物官网

陶术生物

联系我们

电话：

400-820-0310

邮箱：

service@tsbiochem.com

QQ：

2881945090（终端用户）

2885109491（经销商）

经销：

终端：

地址：

上海市静安区江场三路238号8楼