Carbonic Anhydrase 12 Protein, Human, Recombinant (His)

Carbonic Anhydrase 12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.6 kDa and the accession number is O43570-1.

规格	价格/CNY		数量
50 μg	￥ 2,900	现货
100 μg	￥ 4,860	5日内发货
200 μg	￥ 8,150	5日内发货
500 μg	￥ 16,100	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 2,900

现货

100 μg

￥ 4,860

5日内发货

200 μg

￥ 8,150

5日内发货

500 μg

￥ 16,100

5日内发货

生物活性	Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
产品描述	Carbonic Anhydrase 12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.6 kDa and the accession number is O43570-1.

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	O43570-1
别名	HsT18816, FLJ20151, carbonic anhydrase XII, CA12, CAXII
蛋白构建	extracellular domain of Human CA12 (NP_001209.1) (Met 1-Gln 291)
蛋白纯度	≥ 97 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.
分子量	31.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.