ART4/CD297 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.7 kDa and the accession number is Q9CRA0.
生物活性 | Testing in progress |
产品描述 | ART4/CD297 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.7 kDa and the accession number is Q9CRA0. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | Q9CRA0 |
别名 | 4432404K01Rik, DOK1, ADP-ribosyltransferase 4 (Dombrock blood group), ARTC4, DO |
蛋白构建 | The Mouse Art4 (NP_080915.1) (Met1-Lys263) was expressed, fused with the Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 91 % as determined by SDS-PAGE |
分子量 | 53.7 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. |
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ART4/CD297 Protein, Mouse, Recombinant (hFc) 4432404K01Rik DOK1 ADP-ribosyltransferase 4 (Dombrock blood group) DOK 1 DOK-1 ARTC-4 ARTC4 ADP-ribosyltransferase 4 Dombrock blood group ARTC 4 DO recombinant recombinant-proteins proteins protein