Argonaute-2 Protein, Mouse, Recombinant (His)

Argonaute-2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 99 kDa and the accession number is Q8CJG0.

规格	价格/CNY		数量
20 μg	￥ 1,600	现货
100 μg	￥ 5,530	5日内发货
200 μg	￥ 9,430	5日内发货
500 μg	￥ 19,080	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 1,600

现货

100 μg

￥ 5,530

5日内发货

200 μg

￥ 9,430

5日内发货

500 μg

￥ 19,080

5日内发货

生物活性	Testing in progress
产品描述	Argonaute-2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 99 kDa and the accession number is Q8CJG0.

种属	Mouse
表达系统	Baculovirus Insect Cells
标签	N-His
蛋白编号	Q8CJG0
别名	mKIAA4215, 1110029L17Rik, 2310051F07Rik, Ago2, argonaute RISC catalytic component 2, ENSMUSG00000072493, KIAA4215, Gerp95, AI225898, AL022874, Gm10365, Eif2c2, AW546247
蛋白构建	The Mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.
蛋白纯度	> 80 % as determined by SDS-PAGE
分子量	99 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.