AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 5,750 | 5日内发货 |
生物活性 | The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
产品描述 | AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 158 kDa and the accession number is P54619&O43741&P54646. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | P54619&O43741&P54646 |
别名 | AMPK, AMPK(α2, β1, γ1) |
蛋白构建 | The Human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; The Human PRKAB1 (O43741) (Met 1-Ile 272), constructed the plasmid 2; The Human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
蛋白纯度 | > 94 % as determined by SDS-PAGE |
分子量 | 158 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 50 mM Tris, 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.5 mM PMSF, 10% gly, 1 mM GSH, pH 7.4. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
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AMPK (G1/B1/A2) Heterotrimer Protein, Human, Recombinant (His & GST) AMPK, AMPK(α2, β1, γ1) recombinant recombinant-proteins proteins protein