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Aminoacylase 1 Protein, Human, Recombinant (His)

产品编号 TMPY-01058

别名: ACY-1, ACY1D, aminoacylase 1, HEL-S-5

Aminoacylase 1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.3 kDa and the accession number is Q03154-1.

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Aminoacylase 1 Protein, Human, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 3,820		5日内发货

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生物活性	Testing in progress
产品描述	Aminoacylase 1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.3 kDa and the accession number is Q03154-1.

种属	Human
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	Q03154-1
别名	ACY-1, ACY1D, aminoacylase 1, HEL-S-5
蛋白构建	The full length of Human ACY1 (NP_000657.1) (Met 1-Ser 408) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	47.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.0, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.

参考文献

1. Sommer A,et al.(2011) The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 1812(6): 685-90.Maceyka M,et al.(2004) Aminoacylase 1 is a sphingosine kinase 1-interacting protein. FEBS Lett. 568(1-3): 30-4. 2. Cook RM,et al.(1993) Human aminoacylase-1. Cloning, sequence, and expression analysis of a chromosome 3p21 gene inactivated in small cell lung cancer. J Biol Chem. 268(23): 17010-7.

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Keywords

Aminoacylase 1 Protein, Human, Recombinant (His) ACY-1 ACY1D aminoacylase 1 ACY1 ACY 1 HEL-S-5 recombinant recombinant-proteins proteins protein

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