14-3-3 epsilon Protein, Mouse, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 56.1 kDa and the accession number is P62259.
生物活性 | Testing in progress |
产品描述 | 14-3-3 epsilon Protein, Mouse, Recombinant (GST) is expressed in E. coli expression system with GST tag. The predicted molecular weight is 56.1 kDa and the accession number is P62259. |
种属 | Mouse |
表达系统 | E. coli |
标签 | N-GST |
蛋白编号 | P62259 |
别名 | AU019196, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon, 14-3-3 ε, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ε |
蛋白构建 | The Mouse YWHAE (P62259) (Met 1-Gln 255) was fused with the GST tag at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 56.1 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 2 mM GSH, 1 mM DTT, pH 8.2. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner. |
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14-3-3 epsilon Protein, Mouse, Recombinant (GST) AU 019196 AU019196 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon AU-019196 14-3-3 ε tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, ε recombinant recombinant-proteins proteins protein