Triclosan (Irgasan) is an antibacterial and antifungal agent.

在生理相关浓度下，用Triclosan处理的癌细胞显著增加了通过肿瘤形成评估的癌细胞群体数量。Triclosan处理的细胞呈现出类间充质样的形态，并减少了细胞间的黏附。这些细胞在降低E-钙黏蛋白的同时，明显上调了EMT标志物，包括N-钙黏蛋白、波形蛋白、蜗牛和鼻涕虫。Triclosan可能通过EMT过程，促进癌细胞在分离状态下的生存和移动能力[2]。

Triclosan 在促进组织纤维化方面发挥病理作用，导致肝癌发生。Triclosan不仅加快了小鼠肝细胞癌（HCC）的发展，还对肝脏损伤的长期影响有所促进。Triclosan促进细胞纤维化和发生炎症[1]。

CV-1 cells are maintained in DMEM supplemented with 10% FBS, seeded in 96-well plates, and transfected with an expression vector containing a specific nuclear receptor and RXR, along with a luciferase reporter containing the appropriate DNA response element. The assay is first validated with known nuclear receptor ligands as positive controls. For the CAR ligand-binding assay, CV-1 cells are transiently transfected with the expression vector containing the Gal4 DNA-binding domain fused with the ligand-binding domain of murine or human CAR and cotransfected with the luciferase reporter plasmid, mh 100-luc. The day after the transfection, the positive compound of each nuclear receptor or TCS(Triclosan) is added and the cells are incubated for an additional 24 h. The luciferase activities are measured and normalized by β-gal activity.(Only for Reference)