Oxybenzone is a UV filter commonly used in tanning and skin protection agents. It is a derivative of benzophenone and is used as an endocrine disrupting chemical that penetrates the placental and blood-brain barriers. It impairs autophagy, alters epigenetic status and disrupts vitamin X-like receptor signaling in apoptotic neuronal cells.

在HEPM细胞培养中，观察到oxybenzone以剂量依赖的方式抑制PGE2的生产[1]。Benzophenones系列化合物 oxybenzone(BP-3)是可增加DNA损伤率的诱变剂。尤其是在阳光照射下，oxybenzone可以直接作为基因毒性化合物，或通过细胞色素P450酶生物活化成为基因毒性化合物。外用oxybenzone且经UV光照射后，会产生潜在的诱变剂——活性氧种[2]。

在小鼠研究中，Oxybenzone (BP-3)影响小鼠的繁殖能力，诱导哺乳期母鼠的不明原因死亡。小鼠和大鼠的实验均表明，接触Oxybenzone (BP-3)会减少体重，增加肝脏([50 %)和肾脏重量，诱发前列腺重量增加30%，减弱免疫力，增加青少年的子宫重量。在哺乳动物中，Oxybenzone (BP-3)因其具有雌激素和抗雄激素活性而闻名，它能激活雌激素受体蛋白并抑制雄激素受体[2]。

Cells are grown at 37℃ with 100% humidity and 10% CO2. Prior to analyses, cells are subcultured onto 24-well plates and allowed to become 70-90% confluent. On day 1 of the experiment, cells are washed three times with serum-free media containing 0.5% bovine serum albumin (BSA), followed by replacement with the same media that also contained 1 ng/ml of IL-1β with or without oxybenzone. After an exposure time of 24 h, the media are removed, the pH adjusted to 3.5 with HCl, and finally assayed for PGE2-production. (Only for Reference)