LM22A-4 is a brain-derived neurotrophic factor (BDNF) mimetic and agonist of the receptor tropomyosin-related kinase B (TrkB; IC50 : 47 nM in a fluorescence anisotropy assay)

TrkB:47 nM

在体外实验中，LM22A-4通过激活TrkB以及下游的Akt和ERK促生存信号通路，增加了海马神经元的存活率[1]。

将Het小鼠用LM22A-4处理4周，能够在延髓和桥脑中恢复TrkB磷酸化的野生型水平，并且恢复了野生型的呼吸频率[1]。

Wt and Het littermates were divided into 4 groups: Wt vehicle-treated (100 μl of 0.9% NaCl, i.p., b.i.d.), Wt drug-treated (50 mg/kg LM22A-4 in 0.9% NaCl, i.p., b.i.d.), Het vehicle-treated (100 μl of 0.9% NaCl, i.p., b.i.d.), and Het drug-treated (50 mg/kg LM22A-4 in 0.9% NaCl, i.p., b.i.d.).?Each mouse was treated from 8 to 13 weeks of age.?Whole-body plethysmography was performed during week 12 and animals were subsequently killed and tissues harvested for TrkB Western blots and BDNF ELISA during week[1].