Powder: -20°C for 3 years | In solvent: -80°C for 1 year
2-Bromo-4'-hydroxyacetophenone(PTP Inhibitor I) 是一种PTP1B 的有效抑制剂,其Ki=42 μM。
产品描述 | 2-Bromo-4'-hydroxyacetophenone(PTP Inhibitor I) is a cell-permeable, protein tyrosine phosphatase (PTP) inhibitor that covalently blocks the catalytic domain of the Src homology region 2 domain-containing phosphatase (SHP-1(ΔSH2)) with a Ki value of 43 μM and PTP1B with a Ki value of 42 μM [1]. SHP-1 and PTP1B both have known roles in regulating insulin signaling as well as myeloid and lymphoid cell differentiation, making inhibitors of these phosphatases of interest in diabetes, cancer, allergy, and inflammation research [2]. |
靶点活性 | SHP1:43 μM (Ki, cell free), PTP1B:42 μM (Ki, cell free) |
细胞实验 | Cell lines: human B cells. Concentrations: 30, 100, 300, 1000 μM. Method: After incubation with varying concentrations of PTP Inhibitor I for 3 min, the cells are lysed, and the cellular proteins are separated on an SDS-PAGE gel, followed by western blot analysis. |
别名 | SHP-1 Inhibitor II, PTP Inhibitor I, α-Bromo-4-hydroxyacetophenone, 4-Hydroxyphenacyl bromide, 2-溴-4'-羟基苯乙酮 |
分子量 | 215.04 |
分子式 | C8H7BrO2 |
CAS No. | 2491-38-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 20 mg/mL
H2O: Insoluble
DMSO: 60 mg/mL (279.02 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.6503 mL | 23.2515 mL | 46.503 mL | 116.2574 mL |
5 mM | 0.9301 mL | 4.6503 mL | 9.3006 mL | 23.2515 mL | |
10 mM | 0.465 mL | 2.3251 mL | 4.6503 mL | 11.6257 mL | |
20 mM | 0.2325 mL | 1.1626 mL | 2.3251 mL | 5.8129 mL | |
50 mM | 0.093 mL | 0.465 mL | 0.9301 mL | 2.3251 mL | |
100 mM | 0.0465 mL | 0.2325 mL | 0.465 mL | 1.1626 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
2-Bromo-4'-hydroxyacetophenone 2491-38-5 Metabolism Phosphatase SHP-1 Inhibitor II 2 Bromo 4' hydroxyacetophenone alpha-Bromo-4-hydroxyacetophenone a-Bromo-4-hydroxyacetophenone 2Bromo4'hydroxyacetophenone PTP Inhibitor I 4-Hydroxyphenacyl Bromide α-Bromo-4-hydroxyacetophenone 4-Hydroxyphenacyl bromide 2-溴-4'-羟基苯乙酮 inhibit Inhibitor inhibitor