Powder: -20°C for 3 years | In solvent: -80°C for 1 year
(±)-Norcantharidin (Endothall anhydride) 是一种人类结肠癌中 c-Met 和 EGFR 的双重抑制剂,可研究抗癌,具有抗血管生成活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 269 | 现货 | ||
10 mg | ¥ 382 | 现货 | ||
25 mg | ¥ 659 | 现货 | ||
50 mg | ¥ 979 | 现货 | ||
100 mg | ¥ 1,450 | 现货 | ||
500 mg | ¥ 3,630 | 现货 | ||
1 g | ¥ 5,250 | 现货 |
产品描述 | (±)-Norcantharidin (Endothall anhydride) is a synthetic anticancer compound which is a dual inhibitor for c-Met and EGFR in human colon cancers. |
体内活性 | NCTD might be a useful and inexpensive drug candidate to substitute for gefitinib to serve the treatment needs of cancer patients[1].NCTD inhibits the growth of xenografted tumors of human gallbladder carcinoma in nude mice by inducing apoptosis and blocking the cell cycle in vivo[2]. |
细胞实验 | Two human colon cancer cell lines, HCT116 and HT29, were used as model systems to investigate the anti-cancer molecular mechanism of NCTD. Cell cycle arrest and early/late apoptosis were analyzed by flow cytometry. The levels of EGFR, phospho-EGFR, c-Met, phospho-c-Met and other related proteins were quantified by western blot analysis[1] |
动物实验 | The tumor xenograft model of human gallbladder carcinoma in nude mice in vivo was established with subcutaneous GBC-SD cells. The experimental mice were randomly divided into control, 5-FU, NCTD, and NCTD+5-FU groups which were given different treatments. Tumor growth in terms of size, growth curve, and inhibitory rate was evaluated. Cell cycle, apoptosis, and morphological changes of the xenografted tumors were assessed by flow cytometry and light/electron microscopy. The expression of the cell cycle-related proteins cyclin-D1 and p27 as well as the apoptosis-related proteins Bcl-2, Bax, and survivin were determined by the streptavidin-biotin complex (SABC) method and RT-PCR[2]. |
别名 | Endothall anhydride, 去甲斑蝥素, Norcantharidin |
分子量 | 168.15 |
分子式 | C8H8O4 |
CAS No. | 29745-04-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (356.82 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.9471 mL | 29.7354 mL | 59.4707 mL | 148.6768 mL |
5 mM | 1.1894 mL | 5.9471 mL | 11.8941 mL | 29.7354 mL | |
10 mM | 0.5947 mL | 2.9735 mL | 5.9471 mL | 14.8677 mL | |
20 mM | 0.2974 mL | 1.4868 mL | 2.9735 mL | 7.4338 mL | |
50 mM | 0.1189 mL | 0.5947 mL | 1.1894 mL | 2.9735 mL | |
100 mM | 0.0595 mL | 0.2974 mL | 0.5947 mL | 1.4868 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(±)-Norcantharidin 29745-04-8 Angiogenesis JAK/STAT signaling Tyrosine Kinase/Adaptors EGFR c-Met/HGFR Endothall anhydride (±) Norcantharidin (±)Norcantharidin 去甲斑蝥素 Norcantharidin Inhibitor inhibitor inhibit